کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7231214 | 1470954 | 2016 | 22 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A self-powered amperometric lactate biosensor based on lactate oxidase immobilized in dimethylferrocene-modified LPEI
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A self-powered amperometric lactate biosensor based on lactate oxidase immobilized in dimethylferrocene-modified LPEI A self-powered amperometric lactate biosensor based on lactate oxidase immobilized in dimethylferrocene-modified LPEI](/preview/png/7231214.png)
چکیده انگلیسی
Lactate is an important biomarker due to its excessive production by the body during anerobic metabolism. Existing methods for electrochemical lactate detection require the use of an external power source to supply a positive potential to the working electrode of a given device. Herein we describe a self-powered amperometric lactate biosensor that utilizes a dimethylferrocene-modified linear poly(ethylenimine) (FcMe2-LPEI) hydrogel to simultaneously immobilize and mediate electron transfer from lactate oxidase (LOx) at the anode and a previously described enzymatic cathode. Operating as a half-cell, the FcMe2-LPEI electrode material generates a jmax of 1.51±0.13 mA cmâ2 with a KM of 1.6±0.1 mM and a sensitivity of 400±20 μA cmâ2 mMâ1 while operating with an applied potential of 0.3 V vs. SCE. When coupled with an enzymatic biocathode, the self-powered biosensor has a detection range between 0 mM and 5 mM lactate with a sensitivity of 45±6 μA cmâ2 mMâ1. Additionally, the FcMe2-LPEI/LOx-based self-powered sensor is capable of generating a power density of 122±5 μW cmâ2 with a current density of 657±17 μA cmâ2 and an open circuit potential of 0.57±0.01 V, which is sufficient to act as a supplemental power source for additional small electronic devices.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 77, 15 March 2016, Pages 26-31
Journal: Biosensors and Bioelectronics - Volume 77, 15 March 2016, Pages 26-31
نویسندگان
David P. Hickey, Russell C. Reid, Ross D. Milton, Shelley D. Minteer,