کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7231286 | 1470954 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An ultrasensitive performance enhanced novel cytochrome c biosensor for the detection of rebaudioside A
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: An ultrasensitive performance enhanced novel cytochrome c biosensor for the detection of rebaudioside A An ultrasensitive performance enhanced novel cytochrome c biosensor for the detection of rebaudioside A](/preview/png/7231286.png)
چکیده انگلیسی
In this study a novel cyctochrome c modified nanocomposite electrochemical biosensor was developed for the electrochemical determination of rebaudioside A in different food samples. The electrode surface was fabricated with graphene oxide assimilated with gold nanoparticles decorated on multiwalled carbon nanotubes/cytochrome c. The developed biosensor exhibited a 10-fold enhancement in the differential pulse voltammetry signal carried out at pH 11.0 in a 0.1 M borate buffer. Under the optimized conditions, Ip (µA) was proportional to the rebaudioside A concentration in the range of 0.001-0.05 mM (R2=0.8308) and 0.075-1.25 mM (R2=0.9920) with a detection limit (S/N=3) of 0.264 µM. Results of this study revealed that cyctochrome c was adsorbed tightly onto the surface of the modified electrode and showed an enzymatic catalytic activity towards the quasi-reversible reduction of rebaudioside A at â0.1 V (vs Ag/AgCl). The direct electron transfer by cytochrome c was further supported by HOMO-LUMO calculations performed at the density functional theory level. Additionally, the molecular docking simulations predicted a stronger binding affinity of rebaudioside A towards cytochrome c, thus supporting their host-guest relationship. The use of novel electrode materials in this study demonstrates the application of the electrochemical biosensor in the food industry.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 77, 15 March 2016, Pages 116-123
Journal: Biosensors and Bioelectronics - Volume 77, 15 March 2016, Pages 116-123
نویسندگان
Ayyappa Bathinapatla, Suvardhan Kanchi, Parvesh Singh, Myalowenkosi I. Sabela, Krishna Bisetty,