کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7231832 | 1470960 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Self-powered competitive immunosensor driven by biofuel cell based on hollow-channel paper analytical devices
ترجمه فارسی عنوان
سیستم ایمنی زنجیره ای رقابتی خود را که توسط سلول های سوخت زیستی هدایت می شود بر اساس دستگاه های تحلیلی مقاله کانال توخالی
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A mediator-less and compartment-less glucose/O2 enzymatic biofuel cell (BFC) was introduced into microfluidic paper-based analytical devices (μ-PADs) that relies on flow in hollow channels with silver nanoparticles/graphene modified paper electrode as the anodic and cathodic substrate, to implement self-powered sensitive carcinoembryonic antigen (CEA) detection. Glucose dehydrogenase (GDH)-gold nanoparticles bioconjugate modified with CEA acted as a biocatalyst for enhancing glucose oxidation in the bioanode, as well as the transducing enzyme for signaling magnification. Similarly, nanoporous PtNi/bilirubin oxidase (BOD) acted as a biocatalyst for enhancing O2 reduction in the biocathode. With an increase in the concentration of CEA, the amount of CEA-Au-GDH bioconjugate on bioanode decreases, thus leading to the lower output of the as-prepared BFC. This proposed BFC-based self-powered immunosensor for CEA possessed largely increased linear detection range from 1 pg mLâ1 to 0.5 μg mLâ1 with a detection limit of 0.7 pg mLâ1. The proposed BFC-based self-powered immunosensor shows high sensitivity, stability, and reproducibility and can become a promising platform for other protein detection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 71, 15 September 2015, Pages 18-24
Journal: Biosensors and Bioelectronics - Volume 71, 15 September 2015, Pages 18-24
نویسندگان
Shuai Li, Yanhu Wang, Shenguang Ge, Jinghua Yu, Mei Yan,