کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7232724 | 1470965 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Biosensor based on glucose oxidase-nanoporous gold co-catalysis for glucose detection
ترجمه فارسی عنوان
بیوسنسور بر اساس گلوکز اکسیداز-کربن نانوپور طلا برای تشخیص گلوکز
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کلمات کلیدی
گلوکز اکسیداز، ایمن سازی، طلا نانوپور، بیوسنسور، گلوکز،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Promoting the electrocatalytic oxidation of glucose is crucial in glucose biosensor design. In this study, nanoporous gold (NPG) was selected for glucose oxidase (GOx) immobilization and glucose biosensor fabrication because of its open, highly conductive, biocompatible, and interconnected porous structure, which also facilitates the electrocatalytic oxidation of glucose. The electrochemical reaction on the surface of the resulting GOx/NPG/GCE bioelectrode was attributed to the co-catalysis effect of GOx and NPG. A surface-confined reaction in a phosphate buffer solution was observed at the bioelectrode during cyclic voltammetry experiments. Linear responses were observed for large glucose concentrations ranging from 50 μM to 10 mM, with a high sensitivity of 12.1 μA mMâ1 cmâ2 and a low detection limit of 1.02 μM. Furthermore, the GOx/NPG/GCE bioelectrode presented strong anti-interference capability against cholesterol, urea, tributyrin, ascorbic acid, and uric acid, along with a long shelf-life. For the detection of glucose in human serum, the data generated by the GOx/NPG/GCE bioelectrode were in good agreement with those produced by an automatic biochemical analyzer. These unique properties make the GOx/NPG/GCE bioelectrode an excellent choice for the construction of a glucose biosensor.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 66, 15 April 2015, Pages 350-355
Journal: Biosensors and Bioelectronics - Volume 66, 15 April 2015, Pages 350-355
نویسندگان
Chao Wu, Huihui Sun, Yufei Li, Xueying Liu, Xiaoyu Du, Xia Wang, Ping Xu,