کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7232725 | 1470964 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of the automated circulating tumor cell recovery system with microcavity array
ترجمه فارسی عنوان
توسعه سیستم بازیابی خودکار سلول های تومور با آرایش میکروکوزیته
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کلمات کلیدی
سیستم خودکار، سلول های تومور گردش خون، خون، سرطان ریه،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Circulating tumor cells (CTCs) are well recognized as useful biomarker for cancer diagnosis and potential target of drug discovery for metastatic cancer. Efficient and precise recovery of extremely low concentrations of CTCs from blood has been required to increase the detection sensitivity. Here, an automated system equipped with a microcavity array (MCA) was demonstrated for highly efficient and reproducible CTC recovery. The use of MCA allows selective recovery of cancer cells from whole blood on the basis of differences in size between tumor and blood cells. Intra- and inter-assays revealed that the automated system achieved high efficiency and reproducibility equal to the assay manually performed by well-trained operator. Under optimized assay workflow, the automated system allows efficient and precise cell recovery for non-small cell lung cancer cells spiked in whole blood. The automated CTC recovery system will contribute to high-throughput analysis in the further clinical studies on large cohort of cancer patients.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 67, 15 May 2015, Pages 438-442
Journal: Biosensors and Bioelectronics - Volume 67, 15 May 2015, Pages 438-442
نویسندگان
Ryo Negishi, Masahito Hosokawa, Seita Nakamura, Hisashige Kanbara, Masafumi Kanetomo, Yoshihito Kikuhara, Tsuyoshi Tanaka, Tadashi Matsunaga, Tomoko Yoshino,