کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7232778 1644974 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ultrasensitive sandwich-type electrochemical immunosensor based on a novel signal amplification strategy using highly loaded palladium nanoparticles/carbon decorated magnetic microspheres as signal labels
ترجمه فارسی عنوان
با استفاده از یک استراتژی تقویت سیگنال جدید با استفاده از ساندویچ حساسیتی با استفاده از نانوذرات پالادیوم و میکروسکوپ مغناطیسی تزئین شده با کربن به عنوان برچسب سیگنال
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
An ultrasensitive sandwich-type electrochemical immunosensor for quantitative detection of alpha fetoprotein (AFP) was proposed based on a novel signal amplification strategy in this work. Carbon decorated Fe3O4 magnetic microspheres (Fe3O4@C) with large specific surface area and good adsorption property were used as labels to anchor palladium nanoparticles (Pd NPs) and the secondary antibodies (Ab2). Pd NPs were loaded on Fe3O4@C to obtain Fe3O4@C@Pd with core-shell structure by electrostatic attraction, which were further used to immobilize Ab2 due to the bonding of Pd-NH2. A signal amplification strategy was the noble metal nanoparticles, such as Pd NPs, exhibiting high electrocatalytic activities toward hydrogen peroxide (H2O2) reduction. This signal amplification was novel not only because of the great capacity, but also the ease of magnetic separation from the sample solution based on their magnetic property. Moreover, carboxyl-functionalized multi-walled carbon nanotubes (MWCNTs-COOH) were used for the immobilization of primary antibodies (Ab1). Therefore, high sensitivity could be realized by the designed immunosensor based on this novel signal amplification strategy. Under optimal conditions, the immunosensor exhibited a wide linear range of 0.5 pg/mL to 10 ng/mL toward AFP with a detection limit of 0.16 pg/mL (S/N=3). Moreover, it revealed good selectivity, acceptable reproducibility and stability, indicating a potential application in clinical monitoring of tumor biomarkers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 68, 15 June 2015, Pages 757-762
نویسندگان
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