کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7233645 1470977 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A simple and sensitive immunoassay for the determination of human chorionic gonadotropin by graphene-based chemiluminescence resonance energy transfer
ترجمه فارسی عنوان
آزمایش ایمنی ساده و حساس برای تعیین گنادوتروپین کوریونی انسان با استفاده از انتقال انرژی رزونانس شیمیایی مونومرزایی گرافن
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
In this study, we report a strategy of chemiluminescence resonance energy transfer (CRET) using graphene as an efficient long-range energy acceptor. Magnetic nanoparticles were also used in CRET for simple magnetic separation and immobilization of horseradish peroxidase (HRP)-labeled anti-HCG antibody. In the design of CRET system, the sandwich-type immunocomplex was formed between human chorionic gonadotropin (HCG, antigen) and two different antibodies bridged the magnetic nanoparticles and graphene (acceptors), which led to the occurrence of CRET from chemiluminescence light source to graphene. After optimizing the experimental conditions, the quenching of chemiluminescence signal depended linearly on the concentration of HCG in the range of 0.1 mIU mL−1-10 mIU mL−1 and the detection limit was 0.06 mIU mL−1. The proposed method was successfully applied for the determination of HCG levels in saliva and serum samples, and the results were in good agreement with the plate ELISA with colorimetric detection. It could also be developed for detection of other antigen-antibody immune complexes by using the corresponding antigens and respective antibodies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 54, 15 April 2014, Pages 72-77
نویسندگان
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