کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7233804 | 1470983 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
LSI-based amperometric sensor for real-time monitoring of embryoid bodies
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A large scale integration (LSI)-based amperometric sensor is used for electrochemical evaluation and real-time monitoring of the alkaline phosphatase (ALP) activity of mouse embryoid bodies (EBs). EBs were prepared by the hanging drop culture of embryonic stem (ES) cells. The ALP activity of EBs with various sizes was electrochemically detected at 400 measurement points on a Bio-LSI chip. The electrochemical measurements revealed that the relative ALP activity was low for large EBs and decreased with progress of the differentiation level of the ES cells. The ALP activity of the EBs was successfully monitored in real time for 3.5Â h, and their ALP activity in a glucose-free buffer decreased after 2Â h. To the best of our knowledge, this is the first report on the application of an LSI-based amperometric sensor for real-time cell monitoring over 3Â h. The chip is expected to be useful for the evaluation of cell activities.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 48, 15 October 2013, Pages 12-18
Journal: Biosensors and Bioelectronics - Volume 48, 15 October 2013, Pages 12-18
نویسندگان
Mustafa Åen, Kosuke Ino, Kumi Y. Inoue, Toshiharu Arai, Taku Nishijo, Atsushi Suda, Ryota Kunikata, Hitoshi Shiku, Tomokazu Matsue,