کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7234085 | 1470983 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A water-dispersible, ferrocene-tagged peptide nanowire for amplified electrochemical immunosensing
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A ferrocence (Fc)-tagged peptide comprising the phenylalanine-phenylalanine (Phe-Phe) sequence was synthesized and allowed to self-assemble into uniform nanowires with a diameter of ca. 100 nm and lengths in the range of 5-10 µm. The Fc-tagged peptide nanowire (Fc-PNW) become well dispersed in aqueous solution when coated with poly(diallyldimethylammonium chloride) (PDDA). Gold nanoparticles (AuNPs) and antibody molecules can then be adsorbed onto the Fc-PNW surface. The resultant antibody modified Fc-PNW was explored as a detection probe for sensitive electrochemical immunosensing in a sandwich assay wherein the capture antibody was attached onto a graphene/gold nanoparticle (AuNPs-GN) composite film. The as prepared electrochemical immunosensor possesses a low detection limit (5 fg/mL) for human IgG and a wide linear range encompassing four orders of magnitude (from 10 fg/mL to 100 pg/mL). Such a low detection limit stems from the significant signal amplification by the large number of Fc moieties on the PNW (for a 5-µm-long Fc-PNW, the number of Fc moieties is 5Ã105). The electrochemical immunosensor is also highly selective and the sensor was demonstrated to be amenable to real sample analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 48, 15 October 2013, Pages 281-286
Journal: Biosensors and Bioelectronics - Volume 48, 15 October 2013, Pages 281-286
نویسندگان
Yingying Ding, Ding Li, Bin Li, Kai Zhao, Wei Du, Jinyun Zheng, Minghui Yang,