Immunospot assay based on fluorescent nanoparticles for Dengue fever detection

► The immunospot assay for Dengue fever overcomes time limitation problems observed for ELISA tests. ► The procedure requires only 45-60 min and requires only one antibody for detection. ► The method uses 4 μL serum sample and allows multiple sample analysis. ► The detection is based on antibody coated fluorescent nanoparticles. ► The detection limit for Dengue NS1 protein is 5.2 ng/mL and comparable to ELISA tests.