Intraperitoneal nanotherapy for metastatic ovarian cancer based on siRNA-mediated suppression of DJ-1 protein combined with a low dose of cisplatin

We have developed and validated combinatorial treatment for metastatic ovarian cancer which consists of two monotherapies administered sequentially via intraperitoneal route for three cycles: (1) siRNA-loaded nanoparticles aimed to suppress DJ-1 protein, and (2) the chemotherapy agent, cisplatin. DJ-1 suppression by siRNA can simultaneously interfere with the expression of multiple oncogenic proteins responsible for ovarian cancer cells survival, migration, proliferation and resistance to platinum-based drugs. The developed therapy eradicated metastatic ovarian cancer tumors from the mice, and there was no cancer recurrence detected during the duration of the study.103