Stem cell-based gene delivery mediated by cationic niosomes for bone regeneration

The current study presents a novel niosome formulation (DP80) based on 2,3di (tetradecyloxy) propan-1-amine cationic lipid (DTPA) and polysorbate 80 non-ionic tensioactive (P80). The cationic niosomes DP80 were used as vehicles to deliver pUNO1-hBMP-7 plasmid to D1-MSCs. Afterwards, it was confirmed that the delivered hBMP-7 gene was capable of enhancing cell proliferation, increasing ALP activity and inducing spontaneous osteogenic differentiation as observed by deposition of calcified extracellular matrix seen by LM (Von Kossa staining) and TEM. Graphical : Figure 7.107