Aldehyde-functionalized mesostructured cellular foams prepared by copolymerization method for immobilization of penicillin G acylase

• V-MCFs were synthesized by co-condensation of TEOS and VTMS.
• A-MCFs were prepared by copolymerization of V-MCFs with methacrolein.
• A-MCFs retain the ultra-large and continuous 3D mesoporous structure.
• PGA is immobilized covalently on the surface of A-MCFs.
• PGA/A-MCFs shows high initial enzymatic activity and operational stability.

Aldehyde-functionalized mesostructured cellular foams (A-MCFs) were successfully prepared by copolymerization of vinyl groups on the surface of vinyl-functionalized mesostructured cellular foams (V-MCFs) with methacrolein, and used for covalent immobilization of penicillin G acylase (PGA). Effects of functionalization with the organo-functional groups on the physicochemical properties of V-MCFs and A-MCFs, and the enzymatic activity and the operational stability of the immobilized PGA were investigated. A-MCFs-15% sample retains the characteristic ultra-large and continuous 3D mesoporous structure of MCFs with high surface area and large pore volume, which is beneficial for immobilization of PGA and diffusion of substrates and products, and thus results in high enzymatic activity of the immobilized PGA. PGA is immobilized covalently on A-MCFs sample via the reaction to produce Schiff’s base between the free amino groups of lysine residues of PGA and the aldehyde groups on the surface of A-MCFs, which greatly increases the operational stability of the immobilized PGA. PGA/A-MCFs-15% sample shows the initial enzymatic activity of 9531 U g−1 and retains 87% of its initial activity after recycled for ten times.

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