Immobilization of laccase on hairy polymer grafted zeolite particles: Degradation of a model dye and product analysis with MALDI–ToF-MS

• Zeolite particles was grafted with functional poly(2-chloroethyl acrylate).
• Covalent immobilization of laccase was realized on composite particles.
• Stability of laccase was increased upon immobilization on composite particles.
• Dye degradation efficiency of laccase increased upon immobilization.
• The resulting dye products were analyzed by MALDI–ToF-MS.

Poly(2-chloroethyl acrylate), p(CEA), as a functional polymer was grafted via surface initiated atom transfer radical polymerization (SI-ATRP) on zeolite particles. The p(CEA) grafted zeolite particles were aminated, and a ligninolytic enzyme “laccase” from Trametes versicolor was covalently immobilized on the composite particles via glutaraldehyde coupling. The amount of immobilized laccase on the zeolite-g-p(CEA) particles was determined as 17.7 mg g−1 particles. The degradation of a model dye (i.e., Reactive Red 120; RR-120) with the free and immobilized laccases was evaluated in batch and continuous systems. The covalently immobilized laccase showed a high decolorization efficiency toward RR-120 in aqueous solution compared to the free counterpart. The resulting dye degradation products were analyzed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI–ToF-MS).

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