Capillary isoelectric focusing of probiotic bacteria from cow's milk in tapered fused silica capillary with off-line matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identification

- We found method for rapid separation of Lactobacillus in tapered FS capillary.
- CIEF separation capacity and efficiency was increased.
- CIEF was coupled with off-line MALDI-TOF MS identification.
- Method was tested on spiked milk.