کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7560802 | 1491447 | 2014 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Proteomic analysis of cAMP-mediated signaling during differentiation of 3 T3-L1 preadipocytes
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کلمات کلیدی
IBMXPCBIRSEpachydroxymethylglutaryl-CoA synthasecAMP - cAMPHMG-CoA synthase - HMG-CoA سنتازiGFR - IGFRAdipogenesis - آدیپوژنزtwo-dimensional gel electrophoresis - الکتروفورز ژل دو بعدیinsulin receptor substrate - انسولین بستر گیرندهisobutylmethylxanthine - ایزوبویل methylxanthineMyo-inositol - میو-آنزیتولpyruvate carboxylase - پیرووات کربوکسیلازInsulin-like growth factor receptor - گیرنده فاکتور رشد مانند انسولین
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Initiation of adipocyte differentiation is promoted by the synergistic action of insulin/insulin-like growth factor, glucocorticoids, and agents activating cAMP-dependent signaling. The action of cAMP is mediated via PKA and Epac, where at least part of the PKA function relates to strong repression of Rho kinase activity, whereas Epac counteracts the reduction in insulin/insulin-like growth factor signaling associated with complete repression of Rho kinase activity. However, detailed knowledge of the Epac-dependent branch and the interplay with PKA is still limited. In the present study, we present a comprehensive evaluation of Epac-mediated processes and their interplay with PKA during the initiation of 3 T3-L1 preadipocyte differentiation using a combination of proteomics, molecular approaches, and bioinformatics. Proteomic analyses revealed 7 proteins specifically regulated in response to Epac activation, 4 in response to PKA activation, and 11 in response to the combined activation of Epac and PKA during the initial phase of differentiation. Network analyses indicated that the identified proteins are involved in pathways of importance for glucose metabolism, inositol metabolism, and calcium-dependent signaling thereby adding a novel facet to our understanding of cAMP-mediated potentiation of adipocyte differentiation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1844, Issue 12, December 2014, Pages 2096-2107
Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics - Volume 1844, Issue 12, December 2014, Pages 2096-2107
نویسندگان
Kamil Borkowski, Krzysztow Wrzesinski, Adelina Rogowska-Wrzesinska, Karine Audouze, Jesse Bakke, Rasmus Koefoed Petersen, Fawaz G. Haj, Lise Madsen, Karsten Kristiansen,