Preparation and Application of Novel Thermo-sensitive Matrix-based Immobilized Enzyme for Fast and Highly Efficient Proteome Research

A novel immobilized enzyme using soluble thermo-sensitivity polymer as the matrix material was prepared. The thermo-sensitive immobilized trypsin has the feature of “homogeneous digestion in high temperature and heterogeneous separation in low temperature”. It showed the advantage of significantly shortened digestion time and recover & reuse of the enzyme.