کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7589231 | 1492088 | 2016 | 37 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Pressurized liquid extraction of Aglaonema sp. iminosugars: Chemical composition, bioactivity, cell viability and thermal stability
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کلمات کلیدی
PLE1-deoxynojirimycinLMWCDNJIminosugarspNPGDMDPp-nitrophenyl-α-d-glucopyranoside - p-nitrofenyl-a-d-glucopyranosidePressurized Liquid Extraction - استخراج مایع تحت فشارPressurized liquid extraction (PLE) - استخراج مایع تحت فشار (PLE)Cell viability - زنده ماندن سلولgas chromatography–mass spectrometry - طیف سنجی جرم کروماتوگرافی گازα-Glucosidase inhibition - مهار ال-گلوکوزیدازThermal stability - پایداری حرارتیLow molecular weight carbohydrates - کربوهیدراتهای با وزن مولکولی کم
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Pressurized liquid extraction of Aglaonema sp. iminosugars has been optimized. A single cycle under optimal conditions (80 mg, 100 °C, 2 min) was enough to extract ⩾96% of most iminosugars. Further incubation with Saccharomyces cerevisiae for 5 h removed coextracted interfering low molecular weight carbohydrates from extracts of different Aglaonema cultivars. A complete characterization of these extracts was carried out by gas chromatography-mass spectrometry: three iminosugars were tentatively identified for the first time; α-homonojirimycin and 2,5-dideoxy-2,5-imino-d-mannitol were the major iminosugars determined. α-Glucosidase inhibition activity, cell viability and thermal stability of Aglaonema extracts were also evaluated. Extracts with IC50 for α-glucosidase activity in the 0.010-0.079 mg mLâ1 range showed no decrease of Caco-2 cell viability at concentrations lower than 125 μg mLâ1 and were stable at 50 °C for 30 days. These results highlight the potential of Aglaonema extracts as a source of bioactives to be used as functional ingredients.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 204, 1 August 2016, Pages 62-69
Journal: Food Chemistry - Volume 204, 1 August 2016, Pages 62-69
نویسندگان
S. RodrÃguez-Sánchez, A. MartÃn-Ortiz, C. Carrero-Carralero, S. Ramos, M.L. Sanz, A.C. Soria,