کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7599304 1492141 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ultrasound-assisted emulsification-microextraction for the determination of phenolic compounds in olive oils
ترجمه فارسی عنوان
امولسیون-میکرو اکستراسیون با کمک سونوگرافی برای تعیین ترکیبات فنلی در روغن زیتون
کلمات کلیدی
روغن زیتون خام، ترکیبات فنل، امالکسیون املاسیون توسط امواج فراصوتی، کروماتوگرافی مایع
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A reliable, sensitive and effective method based on ultrasound-assisted emulsification-microextraction (USAEME) coupled to HPLC-DAD has been developed to identify and quantify several target phenolic compounds from extra virgin olive oils (EVOO). This approach is based on the emulsification of a microvolume of polar organic extractant in a non-polar liquid sample by ultrasound radiation and further separation of both liquid phases by centrifugation. The percentage of methanol/water (v/v) in the extractant, the volume of extractant, and the extraction time as three effective parameters on the extraction were optimised by a central composite design (Box-Behnken response surface) method. The optimised method presented recoveries in EVOO between 91% and 115% for the target analytes (except vanillin with 65%) and a satisfactory precision with relative standard deviations (RSD%) lower than 8.4% for repeatability and reproducibility. The method showed good linearity and limits of detection and quantification were in the range 0.001-0.14 and 0.004-0.47 mg/kg, respectively. After method validation, it was successfully applied to the analysis of three EVOO samples. All target compounds were detected in all analysed samples. Tyrosol and hydroxytyrosol were the major phenolic compounds, followed by pinoresinol and luteolin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 150, 1 May 2014, Pages 128-136
نویسندگان
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