کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7607254 | 1492985 | 2011 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Pyrolysis-mass spectrometry and gas chromatography-flame ionization detection as complementary tools for soil lipid characterization
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Pyrolysis-mass spectrometry and gas chromatography-flame ionization detection as complementary tools for soil lipid characterization Pyrolysis-mass spectrometry and gas chromatography-flame ionization detection as complementary tools for soil lipid characterization](/preview/png/7607254.png)
چکیده انگلیسی
Lipid biomarker profiles are a powerful tool for assessing soil microbial community structure, but intensive laboratory work and data analysis are needed to construct profiles from phospholipid fatty acids and other common biomarkers. Pyrolysis mass spectrometry (Py-MS) is a alternative method that provides a rapid and sensitive 'fingerprint' of soil lipids and may be sufficient to characterize lipids from various sites. The objective of this work was to evaluate the capacity of pyrolysis metastable atom bombardment time-of-flight mass spectrometry (Py-MAB-TOF-MS) to provide replicable analysis of soil lipids, compared to a routine gas chromatography-flame ionization detection (GC-FID) method. Soils were collected from six agricultural fields under soybean, corn and asparagus production. Soil lipids extracted with 1:2 chloroform:methanol solvent were analyzed with Py-MAB-TOF-MS or transesterified into fatty acids and then analyzed by GC-FID. The two methods were complementary, but distinct: lipid fingerprints, generated from Py-MAB-TOF-MS spectra, included extractable soil lipids from microbial, animal and plant origins plus non-living organic matter in the samples, whereas fatty acid profiles generally represented lipids from soil bacteria and fungi. We conclude that the soil lipid fingerprints generated from Py-MAB-TOF-MS present more variability than lipid biomarker profiles from the GC-FID method because they include a broader group of extractable soil lipids. Further work is needed to identify the molecular fragment masses in Py-MAB-TOF-MS spectra that could precisely identify soil lipids of microbial origin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Analytical and Applied Pyrolysis - Volume 90, Issue 2, March 2011, Pages 232-237
Journal: Journal of Analytical and Applied Pyrolysis - Volume 90, Issue 2, March 2011, Pages 232-237
نویسندگان
R. Jeannotte, C. Hamel, S. Jabaji, J.K. Whalen,