کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7608259 | 1493376 | 2018 | 24 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of new polymer-grafted protein cation exchangers developed by partial neutralization of carboxyl groups derivatized by modification of poly(ethylenimine)-Sepharose with succinic anhydride
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Previously, we have studied protein adsorption and chromatographic behaviors on poly(ethylenimine) (PEI)-grafted Sepharose FF anion-exchange resins, and found that protein uptake rates increased greatly when PEI grafting density reached over a critical ionic capacity (cIC) due to the occurrence of the “chain delivery” effect. Moreover, by partial charge neutralization of starting resin FF-PEI-L740 (ICâ¯=â¯740â¯mmol/L, larger than the cIC) with sodium acetate to FF-PEI-R440, it exhibited a three-fold increase in uptake rate over FF-PEI-L740. In this work, to take the advantages of PEI and extend the applications of the PEI-grafted resins in cation-exchange chromatography, a series of cation exchangers of five different ICs were developed. First, the charged of FF-PEI-L740 was reversed from positive to negative by reaction with excess succinic anhydride, which created a cation-exchanger with an IC of 970â¯mmol/L (FF-FEI-C970). FF-PEI-C970 was further modified with ethanolamine for partial charge neutralizations, leading to the preparation of four charge-reduced cation exchangers with IC values (inâ¯mmol/L) of 780, 630, 560 and 430, which were denoted as FF-PEI-CR780, âCR630 âCR560 and âCR430, respectively. Protein adsorption and chromatographic behaviors were investigated using lysozyme (Lys) as the model protein. It was found that, the resins of high and moderate IC values (ICâ¯â¥â¯560â¯mmol/L) afforded adsorption capacities up to over 230â¯mg/mL. Besides, the uptake rate, represented by the effective pore diffusivity (De/D0), exhibited significant increase from 0.067 (FF-PEI-C970 and FF-PEI-CR780) to 0.343 (FF-PEI-CR630 and FF-PEI-CR560) and then to 1.035 (FF-PEI-CR430) with decreasing IC. It was considered that decreasing IC led to the decreased protein binding sites (binding strength), which encouraged the occurrence of the “chain delivery” effect. Moreover, the resins of high and moderate IC values, particularly, the resins of moderate IC values (FF-PEI-CR630 and FF-PEI-CR560), presented both high adsorption capacities and uptake kinetics at 0-100â¯mmol/L NaCl. Besides, dynamic binding capacity achieved 150â¯mg/mL for the resins of moderate IC values at 0â¯mmol/L NaCl concentration, and afforded >110â¯mg/mL for the resin of high IC values at 0-100â¯mmol/L NaCl concentration. The results proved the excellent IEC performance of the PEI-derived cation exchangers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1550, 18 May 2018, Pages 28-34
Journal: Journal of Chromatography A - Volume 1550, 18 May 2018, Pages 28-34
نویسندگان
Yangyang Zhao, Xiaoyan Dong, Linling Yu, Yang Liu, Yan Sun,