کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7608332 | 1493378 | 2018 | 26 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Screening of stationary phase selectivities for global lipid profiling by ultrahigh performance supercritical fluid chromatography
ترجمه فارسی عنوان
غربالگری انتخاب های فاز ثابت برای طبقه بندی جهانی چربی با استفاده از کروماتوگرافی سیالات فوق کریستالی
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کلمات کلیدی
طیف سنجی جرمی، لیپیدومیکس، طراحی آزمایش، سرم خون،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Stationary phases containing β-hydroxy amines, i.e. 1-AA, DEA and 2-PIC, yielded strong retention and poor peak shapes of zwitterionic lipids with primary amine groups, such as phosphatidylserines, phosphatidylethanolamines and its lyso forms. The BEH and HSS-C18 columns showed strong retention of polar and nonpolar lipids, respectively. The Diol column retained the majority of major lipid classes and also produced symmetric peaks. In addition, this column also produced the highest resolution within and between major lipid classes. An injection solvent composed of methanol:chloroform (1:2, v:v) and the addition of 20â¯mM ammonium formate in the mobile phase improved chromatographic separation and mass spectrometry detection in comparison to ammonium acetate or absence of additive. Finally, chromatographic and mass spectrometric parameters were optimized for the Diol column using a design of experiments approach. The separation mechanism on the Diol column depended on the lipid functionality and the length and degree of unsaturation of the acyl groups. The developed method could resolve 18 lipid classes and multiple lipids within each class, from blood serum and brain tissue in 11â¯min.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1548, 4 May 2018, Pages 76-82
Journal: Journal of Chromatography A - Volume 1548, 4 May 2018, Pages 76-82
نویسندگان
Said Al Hamimi, Margareta Sandahl, Marina Armeni, Charlotta Turner, Peter Spégel,