کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7608879 | 1493390 | 2018 | 18 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Analysis of underivatised low volatility compounds by comprehensive two-dimensional gas chromatography with a short primary column
ترجمه فارسی عنوان
تجزیه و تحلیل ترکیبات پایین نوآوری با استفاده از کروماتوگرافی گاز جامع دو بعدی با ستون کوتاه
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Comprehensive two-dimensional gas chromatography (GCÃGC) approaches with cryogenic modulation were developed for the qualitative analysis of selected low volatility compounds in raw coffee bean extracts, without derivatisation. The approaches employed short first (1D) and second (2D) dimension columns, specifically a 1D 65% phenyl methyl siloxane column (11 m) and a 2D 5% phenyl methyl siloxane column (1 m), which allowed elution of high molar mass compounds (e.g. > 600 Da). Solutes included hydrocarbons, fatty acids, diterpenes, tocopherols, sterols, diterpene esters, and di- and triacylglycerides. An oven temperature program up to 370 °C was employed. The effects of experimental conditions were investigated, revealing that the GCÃGC results strongly depended on the cryogenic trap T, and oven T program. An appropriate condition was selected and further applied for group type analysis of low volatility compounds in green Arabica coffee beans. Retention indices were compiled for 1D GC analysis and were similar for the composite column data in GCÃGC. The elution of some compounds was confirmed by use of authentic standards. The approach allowed direct analysis of coffee extract in ethyl acetate solution, with improved analyte peak capacity (approximately 200 compounds were detected) without prior fractionation or pre-treatment of the sample. This avoided potential hydrolysis of high molar mass conjugate esters as well as degradation of thermally labile compounds such as the derivatives of the diterpenes cafestol and kahweol.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1536, 9 February 2018, Pages 75-81
Journal: Journal of Chromatography A - Volume 1536, 9 February 2018, Pages 75-81
نویسندگان
Fábio Junior Moreira Novaes, Chadin Kulsing, Humberto Ribeiro Bizzo, Francisco Radler de Aquino Neto, Claudia Moraes Rezende, Philip John Marriott,