کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7609293 1493395 2018 25 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid method for the quantification of 13 sulphonamides in milk by conventional high-performance liquid chromatography with diode array ultraviolet detection using a column packed with core-shell particles
ترجمه فارسی عنوان
روش سریع برای اندازه گیری 13 سولفونامید در شیر با استفاده از کروماتوگرافی مایع با عملکرد بالا با عملکرد بالا با استفاده از ستون دایره ای فرابنفش با استفاده از ستون بسته بندی شده با ذرات هسته پوسته
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
In the present study, a column packed with core-shell particles was used for the separation and the quantification of 13 sulphonamides in milk by conventional high-performance liquid chromatography coupled with diode array ultraviolet detection (HPLC/UV-DAD). Preliminary experiments were carried out to investigate selectivity of different stationary phases. Best results were achieved using a C18 column packed with 2.6 μm core-shell particles (diameter 4.6 mm, length 75 mm). A binary gradient elution based on acetate buffer solution at pH 4.50 and a mixture of methanol acetonitrile 50:50 (v/v) was employed at the flow rate of 1.2 mL min−1 with an injection volume of 6 μL. These chromatographic conditions allowed the efficient separation of 13 sulphonamides in about 8 min. To evaluate the suitability of the method for official control analysis, the most important validation parameters were investigated according to the European Decision 657/2002/EC as established for analysis of drug residues in food. Sulphonamides were recovered from milk samples by a simple and quick preparation procedure consisting of an extraction step with chloroform/acetone and a purification step with n-hexane. Mean recoveries from raw milk ranged between 55% and 86% at the Maximum Residual Limit of 100 μg kg−1, and RSDs% resulted lower than Thompson and Horwitz RSD% reference values for all sulphonamides. The LOQ value (2.7-15 μg kg−1) was low enough to satisfy legal limits suggested by European Regulation 37/2010/EC.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1531, 5 January 2018, Pages 46-52
نویسندگان
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