کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7609333 1493396 2017 35 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Application of enzymatic probe sonication for selenium speciation in animal feeds
ترجمه فارسی عنوان
استفاده از دستگاه سانتریفیوژ پروتئین آنزیمی برای تولید سلنیوم در خوراک حیوانات
کلمات کلیدی
عصاره پروتئین آنزیمی، گونه سلنیوم، خوراک حیوانات، طیف سنجی فلورسانس اتمی،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Enzymatic probe sonication (EPS) was investigated as a novel and alternative technology for the extraction of five Se species SeCys2, MeSeCys, Se (IV), SeMet and Se (VI) from animal feeds. The critical parameters of EPS such as enzyme types, ultrasonic power, sample/enzyme ratio, extraction time and temperature were carefully optimized. Under current conditions, one sample can be extracted in 60s, in contrast to several hours required by the conventional enzymatic methods Moreover, the extraction is performed in pure water, no further temperature control or pH adjustment is needed, and therefore the risk of species interconvertion is drastically reduced or inhibited. The Se species were separated and quantitatively determined by high-performance liquid chromatography-hydride generation atomic fluorescence spectrometry (HPLC-HG-AFS), which allows the separation of organic and inorganic Se species in a single chromatographic run. The newly developed method was successfully applied to the determination of Se species in 18 feed samples collected from markets and local farms. Concerning the feed samples studied, the results of this work suggest that stricter measures should be taken to control the Se-enriched feed supplements in terms of Se species.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1530, 29 December 2017, Pages 51-58
نویسندگان
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