کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7609791 1493403 2017 26 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of a conductivity-based photothermal absorbance detection microchip using polyelectrolytic gel electrodes
ترجمه فارسی عنوان
توسعه یک میکروسکوپ الکترومغناطیسی الکترولیتی مبتنی بر هدایت مبتنی بر فوتوترمال
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The development and application of polyelectrolytic gel electrodes (PGEs) for a microfluidic photothermal absorbance detection system is described. The PGEs are used to measure changes in conductivity based on heat generation by analytes absorbing light and changing the solution viscosity. The PGEs are suitable for direct contact conductivity measurements since they do not degrade with exposure to high electric fields. Both a 2-electrode system with DC voltages and a 3-electrode system with AC voltages were investigated. Experimental factors including excitation voltage, excitation frequency, laser modulation frequency, laser power, and path length were tested. The limits of detection for the 3-electrode and 2-electrode systems are 500 nM and 0.55 nM for DABSYL-tagged glucosamine, respectively. In addition, an electrokinetic separation of a potassium, DABSYL-tagged glucosamine, Rhodamine 6G, and Rhodamine B mixture was demonstrated.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1523, 10 November 2017, Pages 140-147
نویسندگان
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