کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7609935 1493471 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Stir-bar supported micro-solid-phase extraction for the determination of polychlorinated biphenyl congeners in serum samples
ترجمه فارسی عنوان
مخلوط کردن نوار با استفاده از استخراج فاز میکرو جامد برای تعیین ترکیبات متخلخل بفنیل در نمونه های سرم
کلمات کلیدی
استخراج فاز میکرو جامد، غشاء کمک استخراج، آماده سازی میکرو اکستراکت و نمونه بیفینیل های پلی کربنات،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
In present work, a new configuration of micro-solid phase extraction was introduced and termed as stir-bar supported micro-solid-phase extraction (SB-μ-SPE). A tiny stir-bar was packed inside the porous polypropylene membrane along with sorbent material and the edges of membrane sheet were heat sealed to secure the contents. The packing of stir-bar inside the μ-SPE device does not allow the device to stick with the wall or any corner of the sample vial during extraction, which is, however, a frequent observation in routine μ-SPE. Moreover, it enhances effective surface area of the sorbent exposed to sample solution through continuous agitation (motion and rotation). It also completely immerses the SB-μ-SPE device in the sample solution even for non-polar sorbents. Polychlorinated biphenyls (PCBs) were selected as model compounds and the method performance was evaluated in human serum samples. After extraction, samples were analyzed by gas chromatography mass spectrometry (GC⿿MS). The factors that affect extraction efficiency of SB-μ-SPE were optimized. Under optimum conditions, a good linearity (0.1⿿100 ng mL⿿1) with coefficients of determinations ranging from 0.9868 to 0.9992 was obtained. Limits of detections were ranged between 0.003 and 0.047 ng mL⿿1. Acceptable values for inter-day (3.2⿿9.1%) and intra-day (3.1⿿7.2%) relative standard deviations were obtained. The optimized method was successfully applied to determine the concentration of PCB congeners in human serum samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1455, 15 July 2016, Pages 37-44
نویسندگان
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