کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7610307 | 1493494 | 2016 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of 2-Aminoacetophenone in wine by high-performance thin-layer chromatography-fluorescence detection
ترجمه فارسی عنوان
تعیین 2 آمینو آستوفنون در شراب با استفاده از عملکرد بالا کروماتوگرافی نازک و تشخیص فلورسنت
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
2-Aminoacetophenone (AAP) is closely correlated with the appearance of the sensory phenomenon of UTA (âuntypical aging off-flavor“) in wine. AAP analyses are generally performed by gas chromatography and mass selective detection (GC/MS), when AAP is extracted from wines by liquid-liquid, solid-liquid or solid phase microextraction. Here we present a rapid, selective and sensitive method for the determination of AAP in wine by high-performance thin-layer chromatography with fluorescence detection (HPTLC-FLD). As internal standard, 2-amino-4-methoxyacetophenone was used. Liquid-liquid extraction with t-butyl methyl ether was followed by a basic cleanup of the extracts, which were applied onto HPTLC amino plates developed with methylene chloride/toluene (7 + 3, v/v) as mobile phase. Dipping the dried plate into hexane-paraffin solution enhanced fluorescence that was scanned at 366/>400 nm. Limits of detection and quantitation were determined to be 0.1 and 0.3 μg Lâ1 wine, respectively, while only AAP concentrations >0.5 μg Lâ1 result in UTA. Recoveries were near 100% for model, white, rosé and red wines. Thus, the HPTLC-FLD method enables the analysis of AAP in wines clearly below the odor thresholds and represents a rapid and convenient screening alternative to existing GC/MS methods.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1432, 5 February 2016, Pages 140-144
Journal: Journal of Chromatography A - Volume 1432, 5 February 2016, Pages 140-144
نویسندگان
Nora Horlacher, Wolfgang Schwack,