Orthogonal separations: Comparison of orthogonality metrics by statistical analysis

A definition of chromatographic orthogonality is suggested that is based on maximizing the values of OMs or OM products. This optimization criterion suggests using the product of a global metric that measures the utilization of separation space (e.g., the convex hull relative area) and a local metric that measures peak spacing (e.g., the box-counting fractal dimension). The “best” column pairs for 2D chromatography are chosen by the product of these OMs.