کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7611404 1493512 2015 15 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An on-line high-performance liquid chromatography-diode-array detector-electrospray ionization-ion-trap-time-of-flight-mass spectrometry-total antioxidant capacity detection system applying two antioxidant methods for activity evaluation of the edible flo
ترجمه فارسی عنوان
یک سیستم تشخیص کروماتوگرافی مایع کروماتوگرافی مایع با عملکرد بالا - دیود آشکارساز - الکترو اسپری یونیزاسیون - طیف سنج یونی - تله - تله - زمان - پرواز - جرم - سیستم تشخیص ظرفیت آنتی اکسیدان با استفاده از دو روش آنتی اکسیدانی برای ارزیابی فعالیت فلو خوراکی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
An on-line high-performance liquid chromatography−diode-array detector−electrospray ionization−ion-trap−time-of-flight−mass spectrometry−total antioxidant capacity detection (HPLC−DAD−ESI−IT−TOF−MS−TACD) system was created for identification and evaluation of antioxidants in Prunus (P.) mume flowers. Applying this system, the HPLC fingerprint, ultraviolet (UV) spectra, mass fragmentations, active profiles against 1,1-diphenylpicryl-2-hydrazyl radical (DPPH
- ) scavenging activity and ferric reducing antioxidant power (FRAP) of each complex sample were obtained simultaneously after one injection. Synchronous structure identification and activities screening of complex samples were thus accomplished. In this study, 78 compounds were identified from P. mume flowers by their chromatographic behaviors, UV spectra and MS data with the assistance of standard compounds and literature reports. The DPPH and FRAP activity of 24 samples (23 different P. mume varieties and 1 related herbal medicine) were then quantified by their detailed activity profiles from the on-line system, and by the total activity of each sample extract from off-line 96-well plate method. As a result, 21 and 32 compounds in the on-line system showed anti-oxidative effects against DPPH and FRAP, respectively. The established on-line system is efficient, sensitive and reliable to tell the DPPH and FRAP antioxidant activities of individual compound in complex samples, and therefore would be a useful and promising technique for antioxidant screening from different food and medicinal matrices.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1414, 2 October 2015, Pages 88-102
نویسندگان
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