کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7611409 1493512 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid separation and identification of phenolics in crude red grape skin extracts by high performance liquid chromatography coupled to diode array detection and tandem mass spectrometry
ترجمه فارسی عنوان
جداسازی سریع و شناسایی فنول ها در عصاره های پوست انگور قرمز خام با استفاده از کروماتوگرافی مایع با کارایی بالا همراه با تشخیص آرایه دیود و طیف سنجی جرم دو طرفه
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A rapid and efficient method was established for the simultaneous determination of structures and configurations for 45 phenolics isolated from crude red grape skin extracts without extensive sample preparation. Separation and compound assignments were achieved using high performance liquid chromatography coupled to diode array detection and tandem mass spectrometry (HPLC-DAD-MS2). A Poroshell 120 EC-C18 (100 mm × 3.0 mm, 2.7 μm) column was employed to separate the phenolics, which were eluted using a gradient of acetonitrile and water acidified with 0.2% formic acid. Phenolics were identified by comparison of their UV-vis spectra, mass spectra and MS2 data with those in the literature. Using this procedure, five compounds were detected for the first time in Vitis amurensis. Good separation of most phenolics was achieved in 26 min. The methods described here can be used for the characterization of phenolics in a variety of grapes and grape products.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1414, 2 October 2015, Pages 138-146
نویسندگان
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