کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7611683 1493518 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of an achiral supercritical fluid chromatography method with ultraviolet absorbance and mass spectrometric detection for impurity profiling of drug candidates. Part II. Selection of an orthogonal set of stationary phases
ترجمه فارسی عنوان
توسعه یک روش کروماتوگرافی مایع باکتریال فوق بحرانی با تشخیص اشعه ماوراء بنفش و طیف سنجی جرمی برای نامطلوب بودن نامزد مواد مخدر. قسمت دوم. انتخاب یک مجموعه متعامد از فازهای ثابت
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The objective of this second part was the selection of an orthogonal set of ultra-high performance stationary phases, that was carried out in two steps. Firstly, a reduced set of analytes (20) was used to screen 23 columns. The columns selected were all 1.7-2.5 μm fully porous or 2.6-2.7 μm superficially porous particles, with a variety of stationary phase chemistries. Derringer desirability functions were used to rank the columns according to retention window, column efficiency evaluated with peak width of selected analytes, and the proportion of analytes successfully eluted with good peak shapes. The columns providing the worst performances were thus eliminated and a shorter selection of columns (11) was obtained. Secondly, based on 160 tested analytes, the 11 columns were ranked again. The retention data obtained on these columns were then compared to define a reduced set of the best columns providing the greatest orthogonality, to maximize the chances to see all impurities within a limited number of runs. Two high-performance columns were thus selected: ACQUITY UPC2 HSS C18 SB and Nucleoshell HILIC.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1408, 21 August 2015, Pages 227-235
نویسندگان
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