کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7611882 1493531 2015 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A systematic approach for avoiding co-detection of oppositely charged analytes in dual-opposite-injection capillary electrophoresis
ترجمه فارسی عنوان
یک روش سیستماتیک برای اجتناب از هماهنگ سازی آنالیت های متضاد متقابل در الکتروفورز مویرگی دو طرفه
کلمات کلیدی
الکتروفورز مویرگی، تزریق دو طرفه، تشخیص همزمان، جداسازی همزمان آنیون ها و کاتیون ها، الکتروپاروگرام شبیه سازی شده، انتخاب الکتروفورز،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Dual-opposite-injection capillary electrophoresis (DOI-CE) is a separation technique that utilizes both ends of the capillary for sample introduction. Depending on the individual electrophoretic mobilities of the analytes of interest and the migration distance that each analyte travels to the detection window, the elution order of analytes in a DOI-CE separation can vary widely. One consequence of a variable elution order is that co-detection of oppositely charged analytes becomes possible under certain experimental conditions. While several approaches currently exist to avoid co-detection, they are often time consuming and inefficient. This paper describes a systematic approach to easily avoid co-detection by analyzing the net mobilities of the analytes of interest to aid in choosing appropriate capillary lengths for a particular separation. Simulated electropherograms that highlight various aspects of this new approach are shown. An electropherogram from the literature is replicated and the derived relationship is applied to show the potential advantages of this new approach.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1395, 22 May 2015, Pages 180-189
نویسندگان
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