کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7611886 1493537 2015 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simultaneous determination of a variety of endocrine disrupting compounds in carrot, lettuce and amended soil by means of focused ultrasonic solid-liquid extraction and dispersive solid-phase extraction as simplified clean-up strategy
ترجمه فارسی عنوان
تعیین همزمان انواع ترکیبات غشایی غدد درون ریز در هویج، کاهو و خاک اصلاح شده با استفاده از استخراج جامد مایع متمرکز اولتراسونیک و استخراج جامدات پراکنده به عنوان یک روش ساده تصفیه
کلمات کلیدی
ترکیب غدد درون ریز، سبزیجات، کمپوست زمین را اصلاح کرد استخراج جامد مایع فراصوتی متمرکز شده، استخراج فاز جامد فشرده، طیف سنجی جرمی کروماتوگرافی مایع دو طرفه،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The present study is focused on the development of an analytical method based on focused ultrasonic solid-liquid extraction (FUSLE) followed by dispersive solid-phase extraction (dSPE) clean-up and liquid chromatography-triple quadrupole tandem mass spectrometry (LC-MS/MS) optimised for the simultaneous analysis of certain endocrine disrupting compounds (EDCs), including alkylphenols (APs), bisphenol A (BPA), triclosan (TCS) and several hormones and sterols in vegetables (lettuce and carrot) and amended soil samples. Different variables affecting the chromatographic separation, the electrospray ionisation and mass spectrometric detection were optimised in order to improve the sensitivity of the separation and detection steps. Under the optimised extraction conditions (sonication of 5 min at 33% of power with pulse times on of 0.8 s and pulse times off of 0.2 s in 10 mL of n-hexane:acetone (30:70, v:v) mixture using an ice bath), different dSPE clean-up sorbents, such as Florisil, Envi-Carb, primary-secondary amine bonded silica (PSA) and C18, or combinations of them were evaluated for FUSLE extracts before LC-MS/MS. Apparent recoveries and precision in terms of relative standard deviation (RSDs %) of the method were determined at two different fortification levels (according to the matrix and the analyte) and values in the 70-130% and 2-27% ranges, respectively, were obtained for most of the target analytes and matrices. Matrix-matched calibration approach and the use of labelled standards as surrogates were needed for the properly quantification of most analytes and matrices. Method detection limits (MDLs), estimated with fortified samples, in the ranges of 0.1-100 ng/g for carrot, 0.2-152 ng/g for lettuce and 0.9-31 ng/g for amended soil were obtained. The developed methodology was applied to the analysis of 11 EDCs in both real vegetable bought in a local market and in compost (from a local wastewater treatment plant, WWTP) amended soil samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1389, 10 April 2015, Pages 8-18
نویسندگان
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