کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7611913 | 1493543 | 2015 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Preparation, chromatographic evaluation and application of adenosine 5â²-monophosphate modified ZrO2/SiO2 stationary phase in hydrophilic interaction chromatography
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The zirconia-coated silica (ZrO2/SiO2) material was obtained by coupling layer-by-layer (LbL) self-assembly method and sol-gel technology, to take dual advantages of the suitable porous structure of SiO2 and basic resistance of ZrO2. Adenosine 5â²-monophosphate (5â²-AMP) was then self-assembled onto ZrO2/SiO2 via Lewis acid-base interaction, generating 5â²-AMP-ZrO2/SiO2. The chromatographic properties of 5â²-AMP-ZrO2/SiO2 were systemically studied by evaluating the effect of acetonitrile content, pH and buffer concentration in the mobile phase. The results demonstrated that the 5â²-AMP-ZrO2/SiO2 possessed hydrophilic interaction chromatographic (HILIC) property comprising hydrophilic, hydrogen-bonding, electrostatic and ion-exchange interactions. For basic analytes, the column efficiency of ZrO2/SiO2 and 5â²-AMP-ZrO2/SiO2 was superior to the bare ZrO2, and different selectivity was obtained after the introduction of 5â²-AMP. For acidic analytes, good resolution was obtained on 5â²-AMP-ZrO2/SiO2 while the analysis failed on the bare ZrO2 column owing to strong adsorption. Hence, the proposed 5â²-AMP-ZrO2/SiO2 had great potential in analyzing acidic compounds in HILIC mode. It was an extended application of ZrO2 based SP.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1383, 27 February 2015, Pages 58-69
Journal: Journal of Chromatography A - Volume 1383, 27 February 2015, Pages 58-69
نویسندگان
Qing Wang, Zhi-Yuan Luo, Mao Ye, Yu-Zhuo Wang, Li Xu, Zhi-guo Shi, Lanying Xu,