Evaluation of preparative hydrodynamic chromatography of silica stationary phase supports

Reducing the particle size distribution (PSD) of sub-2 μm chromatographic packing materials can improve the performance of capillary UHPLC columns, but several size refinement methods are only partially effective in this size range. To this end, a preparative scale hydrodynamic chromatography (HDC) method was developed to size-refine C18 functionalized sub-2 μm particles, but suffered from poor reproducibility and particle aggregation issues. Presented here are improvements based on the use of an ammonium hydroxide as the mobile phase. This mobile phase makes the method reproducible, decreases column conditioning requirements, and focuses on the preparation of bare silica material which allows for a wider variety of stationary phase bondings. Additionally, particle recovery for both non-porous silica size standards and bridged-ethyl hybrid (BEH) particles are detailed to highlight the advantages of this method. The data presented demonstrates the capability of this method to reduce the relative standard deviation (RSD) of the PSD of BEH particles by 33% in under 2 h with sufficient yield to pack several capillary columns.