کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7612603 1493564 2014 36 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simultaneous analysis for water- and fat-soluble vitamins by a novel single chromatography technique unifying supercritical fluid chromatography and liquid chromatography
ترجمه فارسی عنوان
تجزیه و تحلیل همزمان برای ویتامین های محلول در آب و چربی با استفاده از یک روش کروماتوگرافی تک کریستالی، یکپارچه سازی کروماتوگرافی مایع فوق بحرانی و کروماتوگرافی مایع
کلمات کلیدی
کروماتوگرافی یکپارچه، کروماتوگرافی مایع فوق بحرانی، طیف سنجی جرمی، ویتامین های محلول در چربی، ویتامین های محلول در آب،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Chromatography techniques usually use a single state in the mobile phase, such as liquid, gas, or supercritical fluid. Chromatographers manage one of these techniques for their purpose but are sometimes required to use multiple methods, or even worse, multiple techniques when the target compounds have a wide range of chemical properties. To overcome this challenge, we developed a single method covering a diverse compound range by means of a “unified” chromatography which completely bridges supercritical fluid chromatography and liquid chromatography. In our method, the phase state was continuously changed in the following order; supercritical, subcritical and liquid. Moreover, the gradient of the mobile phase starting at almost 100% CO2 was replaced with 100% methanol at the end completely. As a result, this approach achieved further extension of the polarity range of the mobile phase in a single run, and successfully enabled the simultaneous analysis of fat- and water-soluble vitamins with a wide log P range of −2.11 to 10.12. Furthermore, the 17 vitamins were exceptionally separated in 4 min. Our results indicated that the use of dense CO2 and the replacement of CO2 by methanol are practical approaches in unified chromatography covering diverse compounds. Additionally, this is a first report to apply the novel approach to unified chromatography, and can open another door for diverse compound analysis in a single chromatographic technique with single injection, single column and single system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1362, 3 October 2014, Pages 270-277
نویسندگان
, , ,