کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7612763 | 1493568 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Single-step approach for fabrication of vancomycin-bonded silica monolith as chiral stationary phase
ترجمه فارسی عنوان
یک رویکرد تک مرحله ای برای ساختن سیلیکا مونولیت وانکومایسین پیوند شده به عنوان فاز ثابت کریال
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A vancomycin-bonded silica monolithic column for capillary electrochromatography (CEC) was prepared by a single-step in situ sol-gel approach. This sol-gel process incorporates a synthetic sol-gel precursor which contains a macrocyclic antibiotic, vancomycin, to form a porous silica network inside a fused-silica capillary. To avoid degradation of vancomycin during the column fabrication, a mild step was adopted into the sol-gel process. The performance of the vancomycin chiral stationary phase was investigated by CEC in both the reversed-phase mode and the normal-phase mode. The vancomycin chiral stationary phase was optimized with respect to vancomycin loading in the reversed-phase mode for chiral separation of thalidomide enantiomers. The best efficiency and resolution values of 94 600 plates/m and 5.79, respectively, were achieved. The optimized column was further applied to chiral separation of alprenolol enantiomers. A plate height of less than 7 μm for the first eluted enantiomer of alprenolol was obtained in an aqueous mobile phase at a flow rate of 0.74 mm/s. Using enantiomers of seven β-blockers and some other basic enantiomers as test analytes, separation efficiencies of up to 148 100 plates/m in the reversed-phase mode and up to 138 100 plates/m in the normal-phase mode were achieved.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1358, 5 September 2014, Pages 208-216
Journal: Journal of Chromatography A - Volume 1358, 5 September 2014, Pages 208-216
نویسندگان
Ming-Lung Hsieh, Lai-Kwan Chau, Yung-Son Hon,