کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7613932 1493605 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ion-pair ultra-high performance liquid chromatographic analysis of monoamines: Peak-splitting at high flow rates
ترجمه فارسی عنوان
تجزیه و تحلیل کروماتوگرافی مایع منوآمینی فوق العاده با عملکرد یون جفت: تقسیم قله با سرعت جریان بالا
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The use of ion-pair ultra-high performance liquid chromatography (UHPLC) coupled with electrochemical detection (ECD) is of great interest for the fast and sensitive determination of the monoamine neurotransmitters dopamine, noradrenaline and serotonin in microdialysis samples. However, when applying high flow rates in ion-pair UHPLC, other peaks than the initial compound peaks appear on the chromatogram. This peak-splitting phenomenon is caused by disturbed ion-pair retention mechanisms. The influence of several chromatographic parameters is investigated. Peak-splitting is delayed to higher flow rates when increasing the concentration of ion-pair reagent or buffering agent in the mobile phase, when decreasing the percentage of organic modifier in the mobile phase, when applying a stationary phase with a smaller amount of packing material or when increasing the separation temperature. One or a combination of these conditions can be applied to analyze the monoamine neurotransmitters using ion-pair UHPLC-ECD at high flow rates.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1321, 20 December 2013, Pages 73-79
نویسندگان
, , , , ,