کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7613959 1493605 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A simple and highly stable free-flow electrophoresis device with thermoelectric cooling system
ترجمه فارسی عنوان
دستگاه الکتروفورز جریان آزاد و ساده با سیستم خنک کننده ترموالکتریک
کلمات کلیدی
الکتروفورز آزاد جریان، محفظه جداسازی، حرارت جول، کولر ترموالکتریک،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Complex assembly, inconvenient operations, poor control of Joule heating and leakage of solution are still fundamental issues greatly hindering application of free-flow electrophoresis (FFE) for preparative purpose in bio-separation. To address these issues, a novel FFE device was developed based on our previous work. Firstly, a new mechanical structure was designed for compact assembly of separation chamber, fast removal of air bubble, and good anti-leakage performance. Secondly, a highly efficient thermoelectric cooling system was used for dispersing Joule heating for the first time. The systemic experiments revealed the three merits: (i) 3 min assembly without any liquid leakage, 80 times faster than pervious FFE device designed by us or commercial device (4 h); (ii) 5 s removing of air bubble in chamber, 1000-fold faster than a normal one (2 h or more) and (iii) good control of Joule heating by the cooling system. These merits endowed the device high stable thermo- and hydro-dynamic flow for long-term separation even under high electric field of 63 V/cm. Finally, the developed device was used for up to 8 h continuous separation of 5 mg/mL fuchsin acid and purification of three model proteins of phycocyanin, myoglobin and cytochrome C, demonstrating the applicability of FFE. The developed FFE device has evident significance to the studies on stem cell, cell or organelle proteomics, and protein complex as well as micro- or nano-particles.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1321, 20 December 2013, Pages 119-126
نویسندگان
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