Plasma low-density lipoprotein immobilized silica as stationary phase in nano-liquid chromatography

► Plasma LDL particles were covalently immobilized onto modified silica particles. ► Capillary columns were packed with LDL immobilized silica particles. ► Stability and lifetime of LDL modified silica columns were studied by nano-LC. ► Nano-LC with LDL immobilized stationary phase was applied to interaction studies. ► Plasma LDLs of the stationary phase were in situ oxidized with copper.