Separation and quantification of microalgal carbohydrates

► Optimization of chromatography for 13 monosaccharides found in algae. ► Accurate quantification of carbohydrates based on acid hydrolysis and chromatography. ► Best resolution found with derivatization-GC and anion exchange chromatography. ► Application to quantification of carbohydrates in 3 algae strains. ► Comparison of algae with terrestrial, lignocellulosic biomass carbohydrates.