کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7614835 | 1493976 | 2018 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Simultaneous UPLC-MS/MS determination of four components of Socheongryong-tang tablet in human plasma: Application to pharmacokinetic study
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The purpose of this study was to develop a method for simultaneous analysis of schizandrin, ephedrine, paeoniflorin, and cinnamic acid as constituents of Socheongryong-tang tablet in human plasma using UPLC-MS/MS. These four components were separated using water containing 0.01% formic acid and methanol as a mobile phase by gradient elution at a flow rate of 0.3â¯mL/min with a HALO-C18 column (2.1â¯mmâ¯Ãâ¯100â¯mm, 2.7â¯Î¼m particle size). Quantitation was performed on a triple quadrupole mass spectrometer employing electrospray ionization technique operated in multiple reaction monitoring mode. Mass transitions were m/z 432.9â¯ââ¯384.1 for schizandrin, 165.8â¯ââ¯148.1 for ephedrine, 525.0â¯ââ¯449.2 for paeoniflorin, 146.8â¯ââ¯102.9 for cinnamic acid, and 340.0â¯ââ¯324.0 for papaverine as internal standard. Liquid-liquid extraction and protein precipitation with ethyl acetate-methanol (1:2, v/v) were used to obtain these four components. Chromatograms showed high resolution, sensitivity, and selectivity without interference by plasma constituents. Calibration curves of schizandrin, ephedrine, paeoniflorin, and cinnamic acid in human plasma ranged from 0.02 to 8â¯ng/mL, 0.5 to 200â¯ng/mL, 0.2 to 80â¯ng/mL, and 1 to 400â¯ng/mL, respectively. Calibration curves of each analyte displayed excellent linearity, with correlation coefficientsâ¯>â¯0.99. For all four components, both intra- and inter-day precisions (CV%) were <5.99%. The accuracy was 99.35-103.30% for schizandrin, 98.48-104.38% for ephedrine, 97.06-103.34% for paeoniflorin, and 99.97-104.36% for cinnamic acid. This analytical method developed in this study satisfied the criteria of international guidance. It could be successfully applied to pharmacokinetic studies of schizandrin, ephedrine, paeoniflorin, and cinnamic acid after oral administration of Socheongryong-tang tablet to humans.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1095, 15 September 2018, Pages 214-225
Journal: Journal of Chromatography B - Volume 1095, 15 September 2018, Pages 214-225
نویسندگان
Seung-Hyun Jeong, Ji-Hun Jang, Seong-Ho Ham, Seung-Jeong Yang, Hea-Young Cho, Yong-Bok Lee,