کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7615036 | 1493982 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Validation of an LC-MS/MS method for simultaneous quantification of venlafaxine and its five metabolites in rat plasma and its application in a pharmacokinetic study
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A sensitive, selective, and reliable LC-MS/MS method was developed and validated for simultaneous quantification of venlafaxine (VEN) and its 5 metabolites (ODV, NDV, NNDDV, OHV and NODDV) in rat plasma. The calibration ranges are 15.0 to 6000â¯ng/mL for VEN, 1.00 to 400â¯ng/mL for ODV, 5.00 to 2000â¯ng/mL for NDV, 1.00 to 400â¯ng/mL for NNDDV, 10.0 to 4000â¯ng/mL for OHV, and 0.200 to 20.0â¯ng/mL for NODDV. Briefly, 50â¯Î¼L of rat plasma was extracted using liquid-liquid extraction (LLE) with methyl tert-butyl ether (MTBE). The analytes were separated on an Agilent SB-Phenyl (50â¯mmâ¯Ãâ¯4.6â¯mm, 3.5â¯Î¼m) column using a binary gradient of 0.1% formic acid in water versus 0.1% formic acid in acetonitrile at a flow rate of 0.8â¯mL/min. The method was validated following FDA guidance for bioanalytical method validation. Validated method was successfully applied to a pharmacokinetic study of VEN orally administered to rats.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volumes 1087â1088, 15 June 2018, Pages 29-35
Journal: Journal of Chromatography B - Volumes 1087â1088, 15 June 2018, Pages 29-35
نویسندگان
Guodong Gu, Michelle Black, Colt Cookson, Anna Fiorella, Yinghe Li, Steven H. Gorman, Ray Bakhtiar,