کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7615364 | 1493990 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of human insulin in dog plasma by a selective liquid chromatography-tandem mass spectrometry method: Application to a pharmacokinetic study
ترجمه فارسی عنوان
تعیین انسولین انسانی در پلاسمای سگ با روش اسپکترومتری جرمی کروماتوگرافی مایع انتخابی: کاربرد در یک مطالعه فارماکوکینتیک
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A simple, sensitive and selective LC-MS/MS method for quantitative analysis of human insulin was developed and validated in dog plasma. Insulin glargine was used as the internal standard. After a simple step of solid-phase extraction, the chromatographic separation of human insulin was achieved by using InertSustain Bio C18 column with a mobile phase of acetonitrile containing 1% formic acid (A)-water containing 1% formic acid (B). The detection was performed by positive ion electrospray ionization in multiple-reaction monitoring (MRM) mode. Good linearity was observed in the concentration range of 1-1000â¯Î¼IU/mL (r2â¯>â¯0.99), and the lower limit of quantification was 1â¯Î¼IU/mL (equal to 38.46â¯pg/mL). The intra- and inter-day precision (expressed as relative standard deviation, RSD) of human insulin were â¤12.1% and â¤13.0%, respectively, and the accuracy (expressed as relative error, RE) was in the range of â7.23-11.9%. The recovery and matrix effect were both within acceptable limits. This method was successfully applied for the pharmacokinetic study of human insulin in dogs after subcutaneous administration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volumes 1077â1078, 1 March 2018, Pages 85-91
Journal: Journal of Chromatography B - Volumes 1077â1078, 1 March 2018, Pages 85-91
نویسندگان
Shiqi Dong, Yong Zeng, Guangli Wei, Duanyun Si, Changxiao Liu,