کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7617468 | 1494070 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Magnetic ligand fishing combination with high-performance liquid chromatography-diode array detector-mass spectrometry to screen and characterize cyclooxygenase-2 inhibitors from green tea
ترجمه فارسی عنوان
ترکیب ماهیگیری لیگاند مغناطیسی با طیف سنجی جرم سنجی کروماتوگرافی مایع کروماتوگرافی با عملکرد بالا برای مهار کننده های سیکلوکوکسیژناز 2 از چای سبز
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Cyclooxygenase-2 (COX-2) inhibitors may be used to efficiently treat inflammation or cancer diseases. In the present study, we established a new screening assay based on magnetic Fe3O4@SiO2-COX-2 ligand fishing combination with high-performance liquid chromatography-diode array detector-mass spectrometry (HPLC-DAD-MSn) to screen and identify COX-2 inhibitors from green tea. Optimized conditions (pH at 7.4, temperature at 30 °C, and incubation time for 30 min) for fishing out COX-2 inhibitors were achieved by testing positive control, celecoxib, with active and inactive COX-2. Notably, immobilized COX-2 showed high stability (remained 94.7% after ten consecutive cycles), reproducibility (RSD < 10% for batch-to-batch evaluation). Finally, eight catechins with COX-2 binding activity were screened in green tea, and their structures were characterized by ultraviolet (UV), accurate molecular weight, diagnostic fragment ions and nuclear magnetic resonance (NMR). Particularly, the COX-2 inhibitory activities of two rare catechins, [(â)-epigallocatechin-3-(3â³-O-methyl)-gallate (3â³-O-methyl-EGCG, IC50 = 0.17 ± 0.03 μM 0.16 ± 0.01), (â)-epicatechin-3-(3â³-O-methyl)-gallate (3â³-O-methyl-ECG, IC50 = 0.16 ± 0.02 μM)], were reported for the first time. The results indicated that the proposed method was a simple, robust and reproducible approach for the discovery of COX-2 inhibitors from complex matrix.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 973, 15 December 2014, Pages 55-60
Journal: Journal of Chromatography B - Volume 973, 15 December 2014, Pages 55-60
نویسندگان
Xu Deng, Shuyun Shi, Simin Li, Tianlun Yang,