کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7617843 | 1494084 | 2014 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of asperosaponin VI and its active metabolite hederagenin in rat tissues by LC-MS/MS: Application to a tissue distribution study
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کلمات کلیدی
ULOQLLOQESIAsperosaponin VIMRMRSDLC–MS/MS - LC-MS / MSMatrix effect - اثر ماتریکس یا اثر زمینهinternal standard - استاندارد داخلیrelative standard deviation - انحراف استاندارد نسبیcollision energy - انرژی برخوردTissue distribution - توزیع بافتupper limit of quantification - حد بالای اندازه گیریlower limit of quantification - حد پایین کمیت سنجیrelative error - خطای مربوطهGastrointestinal - دستگاه گوارشmultiple reaction monitoring - نظارت چندگانه چندگانهHederagenin - هدراژینsodium carboxymethylcellulose - کربوکسی متیل سلولز سدیمquality control - کنترل کیفیتelectrospray ionization - یونیزاسیون الکترو اسپری
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Two high-performance liquid chromatography (HPLC) coupled with electrospray ionization (ESI) mass spectrometry methods were developed and validated for the simultaneous determination of asperosaponin VI (A-VI) and hederagenin (HG) in rats' various tissues. Biological samples were processed with methanol extraction, and glycyrrhetinic acid was chosen as the internal standard (IS). The analytes were separated on a C18 column with two gradient elution programs for different rat tissue samples. The MS/MS detection was carried out by monitoring the transitions of m/z [MH]â 927.5 â 603.4 for A-VI, m/z [MH]â 471.3 â 471.3 for HG and m/z [MH]â 469.4 â 425.4 for the IS, respectively. The lower limits of quantification (LLOQ) for the two analytes in different rat tissues were 2-6 ng/mL, respectively. The methods were successfully applied to a tissue distribution study of A-VI and its active metabolite HG in rats. The results of the tissue distribution study showed that the highest concentration of A-VI was in the gastrointestinal (GI) tract. Besides, A-VI was mainly distributed in lung, liver, fat and ovary. HG was almost undetectable in most tissues except for the GI tract.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 959, 15 May 2014, Pages 22-26
Journal: Journal of Chromatography B - Volume 959, 15 May 2014, Pages 22-26
نویسندگان
Run Zhang, He Zhu, Li Ding, Zhonglin Yang,