Analysis of the extreme diversity of salivary alpha-amylase isoforms generated by physiological proteolysis using liquid chromatography-tandem mass spectrometry

► Rapid and straightforward saliva sample preparation and LC-ESI-MS/MS detection. ► Untargeted analysis detected physiological proteolysis events. ► Extreme proteolytic diversity of salivary alpha-amylase isoforms detected. ► Implications for biomarker discovery, validation and clinical application.