Direct pharmacokinetic analysis of puqietinone by in vivo microdialysis sampling and turbulent-flow chromatography coupled with liquid chromatography-mass spectrometry

► Microdialysis sampling and turbulent-flow chromatography were coupled to LC/MS. ► The system is able to achieve online desalting and concentrating within 1 min. ► Influences of matrix effect and carry-over were eliminated effectively. ► Lifetime of extraction column is much extended. ► The temporal resolution of sampling points is improved by using MD technology.