A sensitive and rapid ultra HPLC-MS/MS method for the simultaneous detection of clopidogrel and its derivatized active thiol metabolite in human plasma

► We developed and validated a novel uHPLC-MS/MS assay for the simultaneous quantification of clopidogrel and its derivatized active metabolite. ► Clinically relevant calibration ranges for clopidogrel (0.01-50 ng/mL) and active metabolite (0.1-150 ng/m) were used. ► Successfully applied to clinical phase I study using 3 subjects.